To demonstrate the correlation between aggregate formation and cell viability, we compared the effect of VAP-B(WT), the P56S mutant, and the P56S/DCC/II mutant on Neuro2a (N2a) cell viability using the MTT assay. We found that the VAP-B(P56S) mutant reduced N2a cell viability as compared to the WT and the P56S/DCC/II mutant. Furthermore, the VAP-B(P56S) mutant markedly attenuated neurite outgrowth of N2a cells after serum deprivation.